Real-Time PCR

Real-Time PCR, or Reverse Transcription Polymerase Chain Reaction, is a lab technique used to amplify and measure DNA or RNA. It allows scientists to detect and quantify genetic material in real-time during the amplification process. By using fluorescent dyes, it monitors the amount of DNA produced after each cycle of heating and cooling, which helps in identifying specific genes or pathogens. This method is widely used in fields like medical diagnostics, research, and forensics because it provides quick and accurate results, making it essential for understanding diseases and developing treatments.