Quantitative RT-PCR

Quantitative RT-PCR (Reverse Transcription Polymerase Chain Reaction) is a laboratory technique used to measure the amount of specific RNA in a sample. It starts by converting RNA into DNA, which is then amplified in cycles, making it easier to study. By using fluorescent markers, scientists can track how much DNA is produced in real time, allowing them to quantify the original RNA. This method is crucial in fields like genetics, medicine, and research, helping to understand gene expression and diagnose diseases such as cancer or viral infections.