Reverse Transcription PCR (RT-PCR)

Reverse Transcription PCR (RT-PCR) is a laboratory technique used to detect and measure RNA in a sample. It involves two main steps: first, the RNA is converted into DNA using an enzyme called reverse transcriptase. Then, the DNA is amplified through a process called polymerase chain reaction (PCR). This amplification allows scientists to create millions of copies of the DNA, making it easier to analyze. RT-PCR is commonly used in research, diagnostics, and to study gene expression, particularly in understanding diseases like COVID-19, where it helps detect the virus’s genetic material.