Molecular cloning
Molecular cloning is a technique used by scientists to create copies of specific DNA fragments. It involves isolating a piece of DNA that contains a gene of interest, inserting it into a small circular DNA molecule called a plasmid, and then introducing this plasmid into bacteria. The bacteria then replicate, producing many copies of the plasmid, which includes the desired DNA. This process allows researchers to study genes, produce proteins, or create genetically modified organisms for research or therapeutic purposes. Essentially, it's a way to reproduce and manipulate genetic material in a controlled environment.
Additional Insights
-
Molecular cloning is a laboratory technique used to create copies of specific DNA fragments. It involves isolating a piece of DNA, often a gene, and inserting it into a vector, typically a small circle of DNA called a plasmid. This plasmid is then introduced into bacteria, which replicate and produce multiple copies of the DNA. The goal is to study the gene, produce a protein, or engineer organisms for research or medical purposes. Essentially, it allows scientists to make and manipulate large amounts of specific genetic material for various applications.