Maxam–Gilbert sequencing

Maxam–Gilbert sequencing is a method used to determine the precise order of nucleotides in DNA. It involves chemically breaking the DNA at specific bases, which creates fragments of varying lengths. These fragments are then separated by size using gel electrophoresis. By analyzing the pattern of these fragments, scientists can read the DNA sequence. Developed in the 1970s, this technique was one of the earliest methods for DNA sequencing but has largely been replaced by newer, faster technologies. It remains significant for understanding genetic information and the study of genetics.