FRET-based assays

FRET-based assays, or Förster Resonance Energy Transfer, are techniques used to study interactions between molecules, often proteins. In this method, two fluorescent tags are attached to the molecules of interest. When one tag (the donor) is excited by light, it can transfer energy to the second tag (the acceptor) if they are close enough. This energy transfer causes the acceptor to emit light at a different color. By measuring this light, scientists can determine if the molecules are interacting, how close they are, and provide insights into cellular processes or disease mechanisms.