FRET sensors

FRET (Förster Resonance Energy Transfer) sensors are tools used in biology to detect when specific molecules or conditions occur inside cells. They work by attaching two tiny fluorescent molecules—called fluorophores—to a target or a sensing region. When the two fluorophores are very close together, energy can transfer from one to the other upon light excitation, producing a detectable signal change. This change indicates the presence or activity of a specific molecule or environmental factor, allowing scientists to monitor cellular processes in real-time with high sensitivity and spatial precision.