single-molecule FRET

Single-molecule FRET (Fluorescence Resonance Energy Transfer) is a technique used to study proteins and other biological molecules at the individual level. It involves attaching two fluorescent dyes to a molecule: one donor dye that emits light when excited, and one acceptor dye that absorbs that light and re-emits it. When the two dyes are in close proximity, energy transfer occurs, indicating they are interacting. By observing the changes in emitted light, researchers can gain insights into molecular dynamics, interactions, and structures in real time, providing valuable information about biological processes.