FRET-based biosensors

FRET-based biosensors use a technique called Förster Resonance Energy Transfer (FRET) to detect molecular interactions or changes inside cells. They consist of two fluorescent molecules—donors and acceptors—that are close together. When the target molecule or event occurs, it changes the distance or orientation between these fluorophores, altering the energy transfer between them. This change affects the colors and intensity of emitted light, allowing scientists to visualize and measure cellular processes like signaling, enzyme activity, or ion levels in real-time, providing valuable insights into biological functions.