Disk diffusion method
The disk diffusion method, also known as the Kirby-Bauer test, is a laboratory technique used to assess the effectiveness of antibiotics against bacteria. In this method, small discs containing antibiotics are placed on a petri dish that has been inoculated with bacteria. As the antibiotics diffuse into the surrounding agar, they inhibit bacterial growth in certain areas. The result is clear zones around the discs, known as inhibition zones. The size of these zones helps determine how sensitive or resistant the bacteria are to the tested antibiotics, guiding treatment decisions for bacterial infections.
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The disk diffusion method, also known as the Kirby-Bauer test, is a laboratory technique used to determine how effective antibiotics are against bacteria. In this method, a petri dish is coated with bacteria, and small paper disks containing different antibiotics are placed on it. As the dish is incubated, the antibiotics diffuse into the surrounding area. If the bacteria are sensitive to a particular antibiotic, a clear zone will appear around that disk, indicating the bacteria cannot grow there. The size of this zone helps scientists assess the effectiveness of the antibiotics for treating infections.