Agar diffusion

Agar diffusion is a laboratory method used to test how well a substance, like an antibiotic, can inhibit the growth of bacteria. In this process, a sample is placed on a gel called agar, which is infused with bacteria. The substance then diffuses or spreads out from the sample into the surrounding agar. If the substance is effective against the bacteria, you’ll see a clear zone, called the zone of inhibition, where bacteria haven’t grown. Measuring this zone helps determine the potency of the substance in stopping bacterial growth.