Shotgun sequencing

Shotgun sequencing is a method used to read and assemble the DNA sequences of an organism. Instead of trying to decode the genome in one long piece, scientists break it into many smaller, random fragments. They then sequence these fragments, which involves determining the order of the DNA building blocks in each piece. After sequencing, computer programs align and overlap the fragments to reconstruct the original full sequence. This approach is efficient for mapping complex genomes, making it possible to study organisms, understand genetic diseases, and advance genetic research and biotechnology.