Sandwich ELISA

Sandwich ELISA is a laboratory technique used to detect and measure specific substances, usually proteins or antibodies, in a sample. It involves placing the sample between two layers of antibodies: one fixed to a surface to "capture" the target substance, and another that binds to a different part of the target, which carries a detectable marker. When the target is present, it "sandwiches" between these two antibodies. Adding a substrate produces a measurable signal, like a color change, indicating the quantity of the target. This method is highly specific and sensitive for diagnostic and research purposes.