photobleaching

Photobleaching is a phenomenon where a fluorescent substance loses its ability to emit light when exposed to intense light over time. This happens because the light damages the molecules in the substance, altering their structure. As a result, they no longer fluoresce or glow. Photobleaching is commonly observed in scientific experiments involving dyes or fluorescent markers, such as in microscopy, where prolonged exposure to light can hinder the observation of samples. Understanding photobleaching is important for researchers to interpret results accurately and optimize their experimental conditions.