Yeast two-hybrid screening

Yeast two-hybrid screening is a laboratory technique used to study protein interactions. Researchers introduce two proteins into yeast cells: one is fused to a "bait" protein that can activate a reporter gene, while the other is fused to a "prey" protein which can bind to the bait. If the bait and prey proteins interact, they trigger the reporter gene, indicating a successful interaction. This method helps scientists identify and understand how proteins communicate in cells, which is crucial for understanding biological processes and disease mechanisms.