stimulated emission depletion (STED)

Stimulated Emission Depletion (STED) is a microscopy technique that allows scientists to achieve super-resolution imaging. It works by using two laser beams: one excites fluorescent molecules in a sample to emit light, while the second beam, shaped like a donut, depletes fluorescence in surrounding areas. This focused depletion creates a very small spot where fluorescence remains. By scanning this setup across the sample, researchers can capture detailed images with much higher resolution than traditional light microscopy. STED is especially valuable in biology, enabling the observation of tiny structures within cells.