single-molecule fluorescence

Single-molecule fluorescence is a technique that allows scientists to observe and study individual molecules by shining a light on them. When these molecules absorb the light, they emit a tiny amount of fluorescent energy that can be detected with sensitive equipment. This method provides detailed insights into the behavior, interactions, and properties of individual molecules, avoiding the averaging effects seen in bulk measurements. It’s like watching a single particle’s dance instead of a crowded room's overall motion, revealing subtle details about molecular function and dynamic processes.