Dideoxy sequencing

Dideoxy sequencing, also known as Sanger sequencing, is a method used to determine the order of nucleotides (building blocks) in DNA. It involves copying the DNA while adding special nucleotides that can stop DNA synthesis when incorporated. By including these terminators in controlled amounts, DNA fragments of varying lengths are produced, each ending at a specific point. These fragments are separated by size, allowing the sequence to be read from the smallest to largest. This technique enables precise reading of genetic information, playing a key role in genetic research and diagnostics.