Bisulfite Sequencing

Bisulfite sequencing is a technique used to study DNA methylation, an important biological process where chemical groups called methyl groups are added to DNA. In this method, DNA is treated with bisulfite, which converts unmethylated cytosine bases into uracil, while methylated cytosines remain unchanged. When the DNA is then sequenced, researchers can distinguish between methylated and unmethylated cytosines based on these changes. This allows scientists to map methylation patterns across the genome, providing insights into gene regulation, development, and disease processes.