454 sequencing

454 sequencing is a method used to determine the exact order of building blocks in DNA. It works by breaking DNA into small pieces and amplifying each piece to make many copies. These copies are then placed in tiny wells, where each nucleotide (DNA’s basic unit) is added one at a time. When a nucleotide is incorporated, it produces a light signal that is recorded. By detecting these light signals, the sequence of bases (A, T, C, G) in the original DNA can be accurately determined. This process allows rapid and detailed analysis of genetic information.