Ultraviolet-Visible Spectrophotometry

Ultraviolet-Visible (UV-Vis) Spectrophotometry is a technique used to measure how much a substance absorbs light in the ultraviolet and visible parts of the spectrum. When light passes through a sample, certain molecules absorb specific wavelengths, causing a reduction in transmitted light. By analyzing these absorption patterns, scientists can identify substances and determine their concentration. This method is widely used in chemistry and biology because it’s quick, non-destructive, and provides valuable information about the structure and amount of molecules in a sample.