Ubiquitin conjugation assays

Ubiquitin conjugation assays are laboratory tests that examine how a small protein called ubiquitin attaches to other proteins inside cells. This process tags proteins for various fates, such as destruction or activity regulation. The assay involves mixing proteins with ubiquitin and enzymes, then detecting the attachment using methods like Western blotting. This helps scientists understand protein regulation mechanisms, cell health, and disease processes like cancer or neurodegeneration by revealing how proteins are modified and managed within the cell.