Two-Photon Microscopy

Two-photon microscopy is an advanced imaging technique that allows scientists to see deep into living tissues, such as brain cells, with high precision. It uses special lasers that emit light at lower energy levels, which only excites the molecules when two photons hit them simultaneously. This focused excitation minimizes damage and allows for imaging deeper within tissue compared to traditional methods. The technique provides detailed, three-dimensional images of living cells and structures, making it valuable for studying biological processes in real time with minimal disturbance.