Two-Hybrid System

The Two-Hybrid System is a laboratory technique used to study how proteins interact with each other. It works by splitting a protein into two parts, each attached to a different, known "reporter" or "bait" protein. When the two "bait" proteins bind or interact inside a cell, they bring the split parts together, activating the reporter. This activation signals that the proteins are interacting, allowing scientists to identify and analyze protein partnerships crucial for understanding biological functions, disease mechanisms, and potential drug targets.