Two-dimensional NMR

Two-dimensional (2D) NMR is an advanced technique used to analyze molecules by observing how atomic nuclei interact within them. Unlike traditional NMR, which provides a single spectrum, 2D NMR creates a two-axis map that reveals relationships between different nuclei, helping to determine the structure and connectivity of complex molecules. This method enhances the ability to distinguish overlapping signals, making it invaluable in chemistry and biochemistry for understanding protein structures, drug design, and molecular interactions. Essentially, 2D NMR provides a detailed "map" of a molecule's internal environment and connections.