Trypan Blue exclusion

Trypan Blue exclusion is a method used to assess cell health and viability. It relies on the fact that live cells have intact cell membranes that prevent the dye from entering, making them appear clear under a microscope. In contrast, dead or damaged cells have compromised membranes, allowing the dye to penetrate, which stains them blue. By adding Trypan Blue to a cell sample and observing under a microscope, scientists can quickly determine the proportion of living versus dead cells, aiding in research and medical diagnostics.