T4 DNA ligase

T4 DNA ligase is an enzyme that facilitates the joining of DNA fragments by creating a strong chemical bond between their sugar-phosphate backbones. Derived from the T4 bacteriophage, it is commonly used in molecular biology to assemble and manipulate DNA molecules, such as during cloning or genetic modification. It works most effectively in the presence of ATP (adenosine triphosphate), providing the energy needed to form the bond. Essentially, T4 DNA ligase acts as a molecular glue, sealing cracks in DNA molecules to create continuous, stable DNA strands for research and biotechnology applications.