standard curve

A standard curve is a graph used in science to determine the concentration of an unknown sample by comparing it to known reference points. Scientists prepare samples with known amounts of a substance and measure their responses, such as color change or signal intensity. These known points form a line or curve on the graph. When an unknown sample is tested, its response is plotted on the graph, and its concentration is inferred from the curve. This method ensures accurate, reliable measurements and is widely used in laboratories for analyzing substances like proteins, chemicals, or enzymes.