SPIM (Selective Plane Illumination Microscopy)

Selective Plane Illumination Microscopy (SPIM) is an advanced imaging technique that captures detailed, three-dimensional images of living specimens, like tissues or small organisms. It does this by shining a thin sheet of light through the sample from the side, illuminating only one specific plane at a time. A camera then captures the image of that plane. By moving the light sheet and taking multiple images at different depths, researchers can reconstruct a complete, high-resolution 3D view of the entire sample. SPIM allows for real-time, gentle imaging with minimal damage, making it ideal for studying live biological processes.