SMRT sequencing

SMRT sequencing, or Single Molecule Real-Time sequencing, is a technique that reads DNA molecules directly as they are being replicated in real-time. It uses tiny spaces called zero-mode waveguides where individual DNA strands are attached and enzymes make them easier to read. By detecting the fluorescence emitted when nucleotides are added during DNA synthesis, SMRT sequencing can determine the sequence of bases (A, T, C, G) quickly and accurately. This method provides long, continuous reads of DNA, making it valuable for detailed genetic analysis and identifying complex genetic variations.