Smart-seq

Smart-seq is a laboratory technique used to analyze gene expression in individual cells by sequencing their entire set of RNA molecules. It allows scientists to understand what genes are active in each cell, providing detailed insights into cell function, development, and differences within a tissue. The method involves extracting RNA from single cells, converting it into DNA, and then sequencing it to identify each gene's activity levels. This approach helps researchers study complex biological processes, identify rare cell types, and understand diseases at a very precise, cell-by-cell level.