Size exclusion chromatography

Size exclusion chromatography is a technique used to separate molecules based on their size. A mixture is passed through a column filled with porous beads. Smaller molecules can enter these pores and take longer paths, while larger molecules bypass them and flow faster. As a result, larger molecules elute (exit the column) first, followed by smaller ones. This method allows scientists to analyze or purify substances by their size, making it useful in fields like biochemistry and pharmaceuticals for studying proteins, polymers, or other complex molecules.