Single-plane illumination microscopy

Single-plane illumination microscopy (SPIM) is an advanced imaging technique that allows scientists to visualize live biological samples in three dimensions with minimal damage. It works by illuminating only a thin, flat section of the sample using a focused sheet of light, which reduces exposure and improves image clarity. A detector captures the emitted fluorescence from that specific plane. By moving the light sheet through the sample, researchers can create detailed 3D images over time. SPIM is valuable for observing dynamic biological processes in real-time, providing insights into how organisms grow, develop, or respond to stimuli.