Single Molecule Real-Time (SMRT) Sequencing

Single Molecule Real-Time (SMRT) Sequencing is a DNA analysis technology that reads individual DNA molecules in real-time. It uses tiny structures called zero-mode waveguides, where a DNA strand is threaded and copied by special enzymes. As each nucleotide (DNA building block) is incorporated, it emits a unique light signal, allowing the system to identify the sequence directly. This method provides long, accurate reads of DNA sequences quickly and without the need for extensive preparation, making it valuable for understanding complex genomes and detecting genetic variations efficiently.