Single-molecule real-time sequencing

Single-molecule real-time (SMRT) sequencing is a technique that reads DNA sequences by observing individual DNA molecules directly. It uses tiny sensors called nanopores or specialized DNA polymerases to detect the addition of each nucleotide (the building blocks of DNA) as it occurs in real time. This method allows for highly accurate, long-read sequences, providing detailed information about complex genomic regions. Because it analyzes molecules one at a time, SMRT sequencing reduces errors caused by averaging signals and enables the discovery of unique genetic features, making it valuable for comprehensive genome analysis.