SIM (Structured Illumination Microscopy)

Structured Illumination Microscopy (SIM) is an advanced imaging technique that enhances the resolution of microscopes beyond traditional limits. It works by projecting patterned light onto the sample and capturing multiple images with different pattern orientations. These images are then computationally combined to reconstruct a highly detailed image, revealing structures at a finer scale than standard microscopes can see. SIM allows scientists to observe intricate biological details in cells and tissues more clearly, enabling better understanding of microscopic processes without damaging the specimen. It effectively doubles the resolution, making it a powerful tool for biological and biomedical research.