RPLC (Reversed-Phase Liquid Chromatography)

Reversed-Phase Liquid Chromatography (RPLC) is a technique used to separate mixtures of compounds based on their chemical properties. In RPLC, the sample is injected into a column packed with a non-polar, or "hydrophobic," stationary phase, while a liquid solvent (mobile phase) flows through it. More hydrophobic (water-repelling) molecules tend to adhere to the stationary phase longer, separating them from less hydrophobic components. As the mobile phase carries the sample through the column, different compounds exit at different times, allowing their identification and analysis. RPLC is widely used in chemistry and biochemistry for analyzing complex mixtures.