Reversed-Phase Chromatography

Reversed-phase chromatography is a technique used to separate and analyze components of a mixture, often in chemical or biological samples. It involves a column filled with a non-polar (hydrophobic) material, while the sample is dissolved in a more polar solvent. As the mixture passes through the column, polar compounds move quickly with the solvent, while non-polar compounds stick to the non-polar material longer. By gradually changing the solvent's composition, different components can be separated based on their affinity for the stationary phase, allowing for identification and quantification of each component.