ratiometric imaging

Ratiometric imaging is a technique used to measure changes in a specific signal, often in biological studies, by comparing the intensity of two different signals simultaneously. This involves using a special dye or marker that emits two different signals depending on conditions like pH or calcium levels. By taking the ratio of these signals, scientists can accurately determine changes in the target property, reducing errors caused by factors like uneven dye distribution or light variability. This method enhances precision and reliability in imaging, providing clearer insights into dynamic processes within cells or tissues.