qPCR (Quantitative Polymerase Chain Reaction)

Quantitative PCR (qPCR) is a laboratory technique used to measure the amount of specific DNA or RNA in a sample. It amplifies targeted genetic sequences while simultaneously monitoring their quantity in real-time through fluorescent signals. As the DNA copies increase, the fluorescence rises, allowing scientists to determine how much of the original genetic material was present. qPCR is highly sensitive and precise, making it valuable for research, diagnostics, and detecting genetic variations, infections, or gene expression levels. It provides quick and accurate quantification, essential for understanding biological processes and disease diagnosis.