Primer design

Primer design involves creating short DNA sequences that match specific regions of a target DNA. These primers act like starting points for DNA copying during processes like PCR (polymerase chain reaction). A good primer is specific to the target, binds strongly, and avoids unwanted areas, ensuring accurate amplification. Proper primer design considers factors like melting temperature, length, and absence of secondary structures to optimize efficiency and specificity, enabling precise detection or analysis of genetic material in research, diagnostics, or medical testing.