PALM (Photoactivated Localization Microscopy)

Photoactivated Localization Microscopy (PALM) is an advanced imaging technique that allows scientists to observe structures at the nanometer scale, far beyond traditional microscopes. It works by tagging molecules with special fluorescent markers that can be activated individually with light. When activated, each marker emits light briefly, and the precise location is calculated based on that emission. Repeating this process many times builds a highly detailed, super-resolution image of tiny structures inside cells. PALM enables visualization of cellular components at the molecular level, enhancing our understanding of biological processes with remarkable precision.