PALM (Photo-Activated Localization Microscopy)

Photo-Activated Localization Microscopy (PALM) is an advanced imaging technique that allows scientists to see extremely small structures, like proteins inside cells, with high precision. It works by using special fluorescent molecules that can be turned on or off with light. By selectively activating a few molecules at a time and precisely pinpointing their location, PALM builds a detailed, high-resolution map of the structures. Repeating this many times allows researchers to construct super-clear images, surpassing traditional microscopy limits, and gaining insights into cellular detail at the nanoscale.