optical diffraction tomography

Optical diffraction tomography (ODT) is a technique that creates detailed, three-dimensional images of tiny objects, like cells, by shining light through them from different angles. As light passes through and around the object, it bends and scatters in ways that reveal its internal structure. By capturing these patterns from multiple perspectives and applying advanced calculations, ODT reconstructs a 3D map showing the object's internal features without needing dyes or stains. This method provides valuable insights into biological samples and materials in a non-invasive and label-free manner.