multiphoton microscopy

Multiphoton microscopy is an advanced imaging technique that allows scientists to see detailed structures inside living tissues with minimal damage. It works by using very focused, high-intensity laser light to excite molecules in the sample simultaneously through multiple photons. Because this process occurs only at the precise focal point, it enables deep tissue imaging with less scattering of light and reduced background noise. This makes multiphoton microscopy especially useful for studying biological processes in real-time within living organisms, providing high-resolution images while preserving the sample’s integrity.