Multi-Photon Microscopy

Multi-photon microscopy is an advanced imaging technique that allows scientists to see deep into living tissues with high resolution. It uses specialized lasers to excite fluorescent molecules within the sample through simultaneous absorption of multiple lower-energy photons, rather than a single high-energy one. This process reduces damage and improves image clarity at deeper layers. Because excitation occurs only at the focal point, it minimizes background noise and enables detailed, three-dimensional views of complex biological structures in real time. This technology is valuable for studying living tissues, such as brain activity or cellular processes, without extensive sample preparation.