live-cell imaging protocols

Live-cell imaging protocols involve using specialized equipment to observe living cells in real-time, allowing scientists to study cellular processes as they happen. This technique typically includes preparing a sample, often using fluorescent markers to highlight specific proteins or structures, and then capturing images using high-resolution microscopes. The goal is to gain insights into cellular behavior, dynamics, and interactions without disturbing the cells significantly. This approach is essential for understanding various biological phenomena, including cell division, migration, and responses to stimuli, contributing to advancements in medicine and biology.