Light sheet fluorescence microscopy (LSFM)

Light sheet fluorescence microscopy (LSFM) is an advanced imaging technique that allows scientists to observe living tissues and organisms in three dimensions with high resolution. It works by illuminating a thin plane of the sample with a sheet of light, which excites fluorescence in specific molecules. A sensitive camera captures images of each illuminated plane, and the process is repeated to create a detailed 3D image. LSFM is valuable for studying biological processes in real-time, such as development, cell behavior, and disease progression, while minimizing damage to the sample.