Kohler Illumination

Köhler illumination is a technique used in microscopy to produce even, bright lighting that enhances image quality. It works by aligning the microscope’s light source, field diaphragm, condenser lens, and aperture diaphragm so that the light rays are uniformly spread across the specimen. This setup minimizes shadows and glare, providing a clear, high-contrast view of the sample. Essentially, it ensures consistent illumination regardless of the sample’s position, improving the accuracy of observations and detailed imaging. Köhler illumination is fundamental for achieving optimal lighting conditions in high-quality microscopy.