Isothermal Titration Calorimetry (ITC)

Isothermal Titration Calorimetry (ITC) is a technique used to measure how two molecules interact by detecting heat changes during binding. In an ITC experiment, one molecule is slowly added to another in a temperature-controlled environment. When they bind, heat is either released or absorbed. The instrument precisely measures these tiny heat changes, providing detailed information about the strength, affinity, and thermodynamics of the interaction. ITC is valuable in fields like drug development and biochemistry because it offers insight into molecular binding behaviors without needing labels or modifications.